Easy Fabrication of Thin Membranes with Through Holes. Application to Protein Patterning

Available online: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.004426

Abstract

Since protein patterning on 2D surfaces has emerged as an important tool in cell biology, the development of easy patterning methods has gained importance in biology labs. In this paper we present a simple, rapid and reliable technique to fabricate thin layers of UV curable polymer with through holes. These membranes are as easy to fabricate as microcontact printing stamps and can be readily used for stencil patterning. We show how this microfabrication scheme allows highly reproducible and highly homogeneous protein patterning with micron sized resolution on surfaces as large as 10 cm2. Using these stencils, fragile proteins were patterned without loss of function in a fully hydrated state. We further demonstrate how intricate patterns of multiple proteins can be achieved by stacking the stencil membranes. We termed this approach microserigraphy.

Masters T, Engl W, Weng ZL, Arasi B, Gauthier N, Viasnoff V (2012) Easy Fabrication of Thin Membranes with Through Holes. Application to Protein Patterning. PLoS ONE7(8): e44261. https://doi.org/10.1371/journal.pone.0044261
Received: April 21, 2012; Accepted: July 31, 2012; Published: August 31, 2012