Actin dynamics modulate mechanosensitive immobilization of E-cadherin at adherens junctions

Nature Cell Biology: https://www.nature.com/ncb/journal/v16/n6/full/ncb2973.html

Abstract

Mechanical stress is increasingly being shown to be a potent modulator of cell–cell junctional morphologies in developmental and homeostatic processes. Intercellular force sensing is thus expected to be an important regulator of cell signalling and tissue integrity. In particular, the interplay between myosin contractility, actin dynamics and E-cadherin recruitment largely remains to be uncovered. We devised a suspended cell doublet assay to quantitatively assess the correlation between myosin II activity and local E-cadherin recruitment. The single junction of the doublet exhibited a stereotypical morphology, with E-cadherin accumulating into clusters of varied concentrations at the rim of the circular contact. This local recruitment into clusters derived from the sequestration of E-cadherin through a myosin-II-driven modulation of actin turnover. We exemplify how the regulation of actin dynamics provides a mechanism for the mechanosensitive response of cell contacts.

W. EnglB. ArasiL. L. YapJ. P. Thiery V. Viasnoff 

Nature Cell Biology 16, 584–591 (2014)

Received 05 November 2013, Accepted 24 April 2014, Published online 25 May 2014

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s