From micro-dishes to micro-niches, a new method for 3D combinatorial control over cell environmental cues.

Full article available here: Microniches a new method for 3D cell culture


The in vitro methods to recapitulate environmental cues around cells are usually
optimized to screen a specific property of the environment (biochemical nature
or rheological property of the extra cellular matrix (ECM), nanotopography) for
its capability to induce defined cell behaviors (lineage commitment, migration).
Approaches that enable to combine different environmental cues in 3D to assess
the biological response of cells to the spatial organization of different biophysical
and biochemical cues are still missing. We demonstrate how the lamination of
through-hole polymeric bio-functionalized membranes can be implemented to
create complex bona fide microniches with differential 3D environmental
properties using exclusively commercially available material. Our approach
enables to create arrays of microniches ranging from single cells to cell
aggregates and biofunctionalized in 3D simultaneously with topographical
featured, protein patterns and structured ECM surrogate with 1 micro-meter
resolution. We demonstrate how these niches can be used to standardized single
cell shape in 3D or to grow cancer cell aggregates in controlled 3D conditions.


Celine Stoecklin 1,6, Wilhelm W. Chen 1,2,6, Richard de Mets 1, Eileen Fong 2,Vincent
Studer 3, Virgile Viasnoff 1,4,5,7

1 Mechanobiology Institute 5A engineering drive 1 117411 Singapore 2 School of Materials Science and Engineering, Nanyang Technological University, N4.1, 50
Nanyang Avenue, Singapore 639798, Singapore
3 CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France 4 CNRS UMI 3639 5A engineering drive 1 117411 Singapore
5 Department of Biological Sciences, National university of Singapore, 14 Science Drive 4
Singapore 117543
6 These authors contributed equally to this work
7 To whom correspondence should be addressed: